INVESTIGATING REDOX POTENTIAL WITH REDOX-SENSITIVE GFP INDICATORS IN REPRODUCTIVE TISSUES OF A. thaliana

Redox homeostasis is a fundamental property of living cells and actively responds to cellular metabolism and external stimuli. In all cells, small molecules capable of thiol-disulphide exchange such as glutathione (GSH) are abundant and are thought to act as buffers in redox equilibrium [1]. Given the importance and the general interest in cellular redox processes, a noninvasive method that detects redox changes within living cells is highly desirable. So far, there is no general approach to the non-destructive measure of the redox state, and although several methods have been proposed for determining redox status in vivo, all have serious limitations.